Table 1.

Summary of the HIV-1 envelopes tested in the different experimental models.

Experimental model	Host cell (HLA-C allele)	Env (tropism/subtype)
HLA-C siRNA silencing and cell fusion assays	HeLa (Cw12)	ADA (R5/B) LAI (X4/B) NDK (X4/D)
gp120/gp41 transient transfection and cell fusion assays	CHO-HLA-C (Cw4)	93MW965 (R5/C) 91US005 (R5/B) 92UG024 (X4/D) NDK (X4/D) J500 (X4/B)
Pseudovirus transductions	293T (Cw7)	pRHPA4259.7 (R5/B) 6535.3 (R5/B) NDK (X4/D) m7NDK (X4/D)

HLA-C silencing was conducted on human cells (HeLa-derived) physiologically expressing HLA-C and stably expressing Env of different strains (ADA, LAI, NDK).

Transient transfections experiments with plasmids encoding different Envs were conducted on non-human CHO cells stably expressing HLA-C to directly compare the effect of HLA-C in the absence of other human MHC class I molecules.

Pseudoviruses were produced in HLA-C silenced 293T cells since this human cell line is the election host for efficient and quantitative production of pseudotyped virus particle. The Envs tested belong to a standard reference panel (NIBSC EVA CFAR ARP2066) except NDK.