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FMN binding to H. pylori flavodoxin. (a) Titration of FMN binding at 25 °C in MOPS 50 mM, pH 7.0 by fluorescence quenching at 525nm. The experimental data is represented by filled circles and its fit by a gray line. (b) ITC experiment in the same conditions as in (a). Due to kinetic effects upon FMN binding in the last part of the titration, the time between injections was increased to 700 s.
