FIGURE 6.

Temperature dependence, at pH 9.0, of the four macroscopic states of H. pylori apoflavodoxin (a) and holoflavodoxin (b): native (continuous line), first thermal intermediate, I₁ (dashed line), the second intermediate, I₂ (dotted line) and the unfolded state (dash-dotted line). The thermodynamic parameters used to calculated the molar fractions of the different states were the ones obtained from DSC analysis (Table 1 and 3). The sequential unfolding model was used both to fit and to calculate the molar fractions.