Table 2. Apoflavodoxin/FMN binding energetics.

Parameter	Protein
	H. pyloria		Anabaena WTb	Anabaena W57Ac
	pH 7	pH 9	pH 7	pH 7
ITC
Kd (nM)	27 ± 1	23 ± 2	-	-
ΔG (kcal/mol)	-10.3 ± 0.1	-10.3 ± 0.1	-	-
ΔH (kcal/mol)	-21 ± 0.3	-25 ± 0.2	-11.8 ± 0.3	-7.1 ± 0.2
ΔS (cal.mol-1.K-1)	-36 ± 1	-49 ± 0.7	-	-
ΔCP (cal.mol-1.K-1)	-600d ± 30	-	-600 ± 20	-620 ± 50
Spectroscopy
Kd (nM)	4.4 ± 0.7	-	0.26 ± 0.06	5.2 ± 0.9 (11.4)
ΔG (kcal/mol)	-11.4 ± 0.5	-	-13.1 ± 0.1	-11.3 ± 0.1 (-10.8)
ΔHe (kcal/mol)	-14 ± 2	-	-	-
ΔS (cal.mol-1.K-1)	-7.7 ± 6	-	8f ± 2	14f ± 1

^aAt 25 °C, in MOPS 50 mM, pH 7.0 or EPPS 50 mM, pH 9.0. The errors reported in this Table were provided by the fitting program and propagated when required.

^bAt 25 °C in MOPS 50 mM, pH 7.0(ref. 7). ΔH and ΔS values were measured in 50 mM phosphate, pH 7.0.

^cAt 25 °C in 50 mM phosphate, pH 7.0 (ref. 7). Values in parenthesis were obtained in 50 mM MOPS, pH 7.0.

^dCalculated as the slope of ΔH vs T at 10, 15, 20 and 25 °C.

^eCalculated from spectroscopic titrations at 11, 15 and 20 °C (K_d = 2.1, 3.4 and 4.4 nM, respectively).

^fΔS in this case was calculated from the ΔG value spectroscopically determined and the calorimetrically measured ΔH value.