Skip to main content

View full-text article in PMC

. Author manuscript; available in PMC: 2009 Jan 15.

Published in final edited form as: Biochemistry. 2007 Dec 21;47(2):627–639. doi: 10.1021/bi701365e

Table 3. Stability of H. pylori holoflavodoxin towards thermal unfolding^a.

Three-state analysis
	1^st Transition			2^nd Transition

	T_m1	ΔH₁		T_m2	ΔH₂
DSC_vH	335	58
Fluorescence	324 ± 1	30 ± 2		341 ± 1	50 ± 2
Far-UV CD	324 ± 0.5	55 ± 1		337 ± 1	65 ± 5
Near-UV CD^c	323.2 ± 0.1	63 ± 1
Visible CD	323.7 ± 0.1	54 ± 1
Four-state analysis
	1^st Transition			2^nd Transition			3^rd Transition

	T_m1	ΔH₁	ΔC_P1	T_m2	ΔH₂	ΔC_P2	T_m3	ΔH₃	ΔC_P3

DSC_cal pH 9	327.2 ± 0.2	43 ± 0.5	1.2	334 ± 1	65 ± 1	1.0	341 ± 0.5	50 ± 1	0.5
Spectr. pH 9^b	325.1 ± 0.5	44 ± 1		331 ± 1	51 ± 4		341 ± 1	40 ± 2
Spectr. pH 7^b	327 ± 3	43 ± 10		335 ± 5	32 ± 10		344 ± 5	43 ± 10

^a

In EPPS 50 mM, pH 9, unless otherwise indicated. Units: T_m(K), ΔH (kcal.mol^-1), ΔC_P (kcal.mol^-1.K^-1)

^b

Parameters obtained from the global fit to equation 1.