a) Hybridization kinetics of MB-1P and MB-4P. Three hybridization trials were conducted for each beacon (a,b,c) [MB]=100nM, [cDNA]=1μM. b) Thermal denaturation profiles of multiple-pyrene labeled beacons. Monomer intensity (376 nm) was monitored for MB-1P (blue) and excimer intensity (480 nm) was monitored for MB-2P (black), MB-3P (red), and MB-4P (green) [MB]=100nM