Figure 4.

(A) Human smooth muscle cell migration to thrombospondin-1 is inhibited by anti-IAP and thrombospondin-1 antibodies. Smooth muscle cells were preincubated with 100 μg/ml C6.7 or 1F7, 2D3 at 37°C for 15 min before being added to the upper compartment of the Boyden chamber. 4N1K (100 μM) was present in the lower compartment, and TBS was used as control. (B) Human smooth muscle cell migration to 4N1K is inhibited by antibodies against IAP and β1 integrin. Smooth muscle cells were preincubated in the presence or absence of β1 mAb (1:1500) or other mAbs as indicated (100 μg/ml) at 37°C for 15 min before being added to the upper compartment of the Boyden chamber.