Figure 1.

Expression of wild-type and constitutively active α-factor receptors. Various myc-tagged α-factor receptors were expressed from their normal promoters on centromere-containing plasmids (pRS314 derivatives) in a ste2Δ mutant (KBY16). Equivalent amounts of protein extract (50 μg) prepared from cells carrying a control plasmid (lane 1), or plasmids expressing wild-type STE2-myc (lane 2) or constitutively active α-factor receptors (ste2P258L-myc, lane 3; ste2P258Y-myc, lane 4) were resolved by SDS-PAGE. Immunoblotting was performed by using 9E10 antibodies specific for the c-myc epitope and a chemiluminescence detection system.