Figure 2.
Luciferase activity in Jurkat pIgR-ζ is stimulated by dIgA and tIgA in a dose-dependent manner. Jurkat pIgR-WT (open symbols) or pIgR-ζ (closed symbols) cells (1 × 107) were transiently transfected with a NFAT-luciferase plasmid. After 24 h, 1 × 105 cells were stimulated with increasing concentrations of monomeric IgA (squares), dIgA (triangles), or tIgA (circles) for 6.5 h. Cell lysates were analyzed for luciferase activity with 1 mM luciferin. The results are representative of three independent experiments. The inset provides the luciferase values for cells exposed to c305 antibody (anti-TCR, 1:500, open diamond) or anti-SC rabbit polyclonal antibody (1:500, closed diamond).