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. 1999 May;10(5):1495–1510. doi: 10.1091/mbc.10.5.1495

Figure 1.

Figure 1

ssp1Δ and spc1Δ cells are hypersensitive to KCl and low pH at 35°C. (A) ssp1Δ and spc1Δ cells are unable to form colonies at low pH. Strains Q868 (wt), Q1528 (ssp1Δ), and KS1366 (spc1Δ) were grown on EMM at 30°C overnight and then streaked onto fresh EMM plates, pH 5.5, or EMM plates, pH 3.5, and incubated overnight at 35°C. (B) High osmolarity and low pH induce mitotic delay or arrest in both ssp1Δ and spc1Δ cells. The same strains as in A were grown on YEA complex medium at 30°C overnight and then streaked onto fresh YEA and YEA + 1 M KCl plates and incubated overnight at 35°C. To assess the effect of low pH, cells were treated as in A. The numbers represent cell sizes at septation in μm ± SD (n > 100).