Figure 2.

SspI and Spc1 act through independent pathways. (A) Strains Q868 (wt), Q1528 (ssp1Δ), KS1366 (spc1Δ), and Q1540 (ssp1Δ spc1Δ) were grown on YEA complex medium at 30°C overnight and then streaked onto fresh YEA + 0.6 M KCl plates and incubated for 24 h at 30°C. (B) Strains Q1521 (cdc25–22^ts), Q353 (wee1–50^ts cdc25–22^ts), and Q1541 (ssp1Δ wee1–50^ts cdc25–22^ts) were picked from overnight 25°C YEA plates, streaked onto fresh YEA plates, and incubated overnight at 35°C. (C) The same strains as in A were grown in liquid YEA cultures for 6 h and then stained with TRITC–phalloidin to visualize filamentous actin.