Table 4.
Mitochondrial inheritance is delayed in ptc1Δ cells after α-factor arrest and release at 25°C
| Strain | Time after release from α-factor (h) | % of unbudded cells | % of smalla budded cells | % of small buds without mitochondria | % of largeb budded cells | % of large buds without mitochondria | % of multiplyc budded cells | % of multiply budded cells without mitochondria in largest bud |
|---|---|---|---|---|---|---|---|---|
| WT | 0.5 | 96 | 4 | 25 | 0 | 0 | ||
| ptc1Δ | 0.5 | 99 | 0 | 0 | 1 | 0 | 0 | |
| WT | 1.0 | 25 | 72 | 9.7 | 3 | 0 | 0 | |
| ptc1Δ | 1.0 | 37 | 59 | 93.2 | 4 | 25 | 0 | |
| WT | 1.5 | 25 | 29 | 3.4 | 46 | 0 | 0 | |
| ptc1Δ | 1.5 | 32 | 48 | 87.5 | 20 | 5 | 0 | |
| WT | 2.0 | 8 | 20 | 10 | 71 | 1.4 | 1 | 0 |
| ptc1Δ | 2.0 | 4 | 23 | 78.3 | 72 | 2.9 | 1 | 0 |
| WT | 2.5 | 16 | 56 | 7.1 | 28 | 0 | 0 | |
| ptc1Δ | 2.5 | 13.9 | 16.8 | 88.2 | 60.4 | 0 | 8.9 | 0 |
| WT | 3.0 | 10 | 56 | 5.4 | 34 | 0 | 0 | |
| ptc1Δ | 3.0 | 8 | 11 | 81.8 | 26 | 7.7 | 55 | 3.6 |
| WT | 3.5 | 41 | 14 | 7.1 | 45 | 0 | 0 | |
| ptc1Δ | 3.5 | 10 | 10 | 80 | 19 | 0 | 61 | 0 |
At least 100 cells were scored for each strain at each time point.
a
Buds less than half the diameter of the mother cell.
b
Buds greater than half the diameter of the mother cell.
c
An increase in the percentage of multiply budded cells is observed in ptc1Δ cells after α-factor treatment. The percentage of unbudded wild-type cells upon α-factor release at 25°C was 99%. The percentage of unbudded ptc1Δ cells on α-factor release at 25°C was 90%.