Table 5.
Mitochondrial inheritance is delayed in ptc1Δ cells after α-factor arrest and release at 30°C
| Strain | Time after release from α-factor (h) | % of unbudded cells | % of smalla budded cells | % of small buds without mitochondria | % of largeb budded cells | % of large buds without mitochondria | % of multiplyc budded cells | % of multiply budded cells without mitochondria in largest bud |
|---|---|---|---|---|---|---|---|---|
| WT | 0.5 | 78 | 22 | 4.5 | 0 | 0 | ||
| ptc1Δ | 0.5 | 84 | 16 | 93.8 | 0 | 0 | ||
| WT | 1.0 | 19 | 43 | 4.7 | 38 | 0 | 0 | |
| ptc1Δ | 1.0 | 26 | 69 | 98.6 | 5 | 40 | 0 | |
| WT | 1.5 | 2 | 9 | 11.1 | 86 | 0 | 3 | 0 |
| ptc1Δ | 1.5 | 7 | 20 | 80 | 73 | 17.8 | 0 | |
| WT | 2.0 | 6 | 27 | 7.4 | 67 | 1.5 | 0 | |
| ptc1Δ | 2.0 | 4 | 12 | 91.7 | 55 | 18.2 | 29 | 3.4 |
| WT | 2.5 | 27 | 13 | 7.7 | 60 | 0 | 0 | |
| ptc1Δ | 2.5 | 4 | 6 | 83.3 | 26 | 7.7 | 64 | 9.4 |
At least 100 cells were scored for each strain at each time point.
a
Buds less than half the diameter of the mother cell.
b
Buds greater than half the diameter of the mother cell.
c
An increase in the percentage of multiply budded cells is observed in ptc1Δ cells after α-factor treatment. The percentage of unbudded wild-type cells upon α-factor release at 30°C was 99%. The percentage of unbudded ptc1Δ cells on α-factor release at 30°C was 98%.