Table 6.

Mitochondrial inheritance is delayed in ptc1Δ cells after α-factor arrest and release at 37°C

Strain	Time after release from α-factor (h)	% of unbudded cells	% of smalla budded cells	% of small buds without mitochondria	% of largeb budded cells	% of large buds without mitochondria	% of multiplyc budded cells	% of multiply budded cells without mitochondria in largest bud
WT	0.5	50	48	22.9	2	0	0
ptc1Δ	0.5	50	45	93.3	5	60	0
WT	1.0	9	78	6.4	13	0	0
ptc1Δ	1.0	12	75	88	13	30.8	0
WT	1.5	34	18	22.2	48	0	0
ptc1Δ	1.5	15.7	30.4	90.3	53.9	41.8	0
WT	2.0	34	31	12.9	35	0	0
ptc1Δ	2.0	15	9	77.8	55	18.2	21	0
WT	2.5	51	35	11.4	14	0	0
ptc1Δ	2.5	16.8	14.9	60	29.7	46.7	38.6	23.1
WT	3.0	19	56	5.4	24	0	1	0
ptc1Δ	3.0	27.3	21.2	76.2	7.1	14.3	44.4	9.1

At least 100 cells were scored for each strain at each time point. 

^a Buds less than half the diameter of the mother cell. 

^b Buds greater than half the diameter of the mother cell. 

^c An increase in the percentage of multiply budded cells is observed in ptc1Δ cells after α-factor treatment. The percentage of unbudded wild-type cells upon α-factor release 37°C was 98%. The percentage of unbudded ptc1Δ cells on α-factor release at 37°C was 88%.