Figure 1.

Maps of Paramecium gene sequences used in microinjection experiments. All sequences were cloned in plasmid vectors as described in MATERIALS AND METHODS. Open boxes, coding sequences; solid boxes, flanking or intron sequences; shaded boxes, coding sequences not included in the construct. Regions used as probes for hybridization are represented as a solid line; it is important to note that the ICL1b, T1b, and T4a probes are subfamily specific: they hybridize, respectively, with all members of the ICL1, T1, and T4 gene subfamilies on Southern blots (Madeddu et al., 1995, 1996). The pND7+ plasmid insert is derived from p201ND7 previously described by Skouri and Cohen (1997).