Figure 8.

Evaluation of plasmid copy number in cells transformed with ND7 sequences. The number of clones as a function of copy number (in haploid genome equivalents) of pND7+ maintained after microinjection of either wild-type or nd7 mutant cells is given. The phenotypes of the clones are also indicated (white background, fewer than 10 trichocysts secreted per cell; lightly shaded background, approximately 100 trichocysts; darkly shaded background, indistinguishable from wild-type control cells; hatched background, no clones encountered). These data are compared with the number of copies of pND7− maintained in clones of wild-type cells. The pND7− data are the same as in Figure 9. In all cases, copy number was determined by a dot blot 10 fissions after injection.