Figure 2.

Cell cycle regulation of PCL2 and PCL9 transcript levels and Pcl9 protein level. A wild-type strain containing a 3 myc epitope-tagged version of the PCL9 gene was arrested using α-factor and reinoculated into fresh medium. Samples were taken 5 min and 15 min after the release from the α-factor holding, and then at 15-min intervals. Samples were analyzed for (A) the percentage of budded cells and cells with divided chromatin; transcript levels of PCL9, SIC1, PCL2, CDC6, PCL1, CLN2, and MET4. Quantitation of transcript levels are relative to MET4; PCL9 (solid circles), SIC1 (open circles), PCL2 (solid diamonds), PCL1 (crosses), CDC6 (triangles). (B) mycPcl9 protein level. Solid circles, PCL9 transcript levels; crosses, Pcl9 protein levels.