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. Author manuscript; available in PMC: 2008 Sep 8.
Published in final edited form as: Biochemistry. 2006 Mar 14;45(10):3219–3225. doi: 10.1021/bi0519497

Figure 3. Titration of the [4Fe–4S]1+ Cluster in the Presence of azaSAM.

Figure 3

(A) 10 K EPR spectra from coulometric titration. Arrows shown are the two magnetic field values used for peak-to-peak intensity determinations. Spectra shown are computed averages of four 2 minute scans with time constant of 0.3 sec. Spectra were recorded at 9.256 GHz, with a modulation amplitude of 16 Gauss, 1mW power, and gain of 8000. (B) Potential vs. peak-to-peak intensity. The best-fit curve of the data to Equation 1 is shown in blue. Signal intensities were normalized for protein concentrations by means of a BCA protein assay (see text).