Figure 1.

Actin staining in JAS-treated cells. FITC-phalloidin labeling on control and JAS-treated polarized MDCK cells. Confocal microscopy of FITC-phalloidin labeling in nontreated MDCK cells (a, c, and e) and cells pretreated with 1 μM JAS for 45 min at 37°C (b, d, and f). Typical patterns of labeling for F-actin in the apical terminal web (a), around the midcell cortical web (c), and in stress fibers at the base of cells (e) were seen in control cells. JAS competed with phalloidin binding sites resulting in less-intense labeling at all levels (b, d, and f) of the cell monolayer. Bar, 10 μm.