Figure 11.

Effects of JAS and nocodazole on FITC-dextran uptake. FITC-dextran uptake was compared in JAS- and nocodazole-treated MDCK cells incubated with FITC-dextran in the basolateral chamber for 15 min. In JAS-treated cells, large characteristic labeled structures were present at the middle (a) and basal (c) region of the monolayer. In nocodazole treated cells, FITC-dextran-labeled compartments were present at the basolateral cell periphery (d). In contrast to JAS-treated cells, there were less labeled compartments in the supranuclear region of nocodazole-treated cells (b), the labeled endocytic vesicles being smaller and more uniform in size (b and d). Bar, 10 μm.