Figure 1.

Specificity of anti-radixin antibodies. (A) DRG neuronal lysates analyzed by immunoblot with rabbit polyclonal antibodies 220 (lane a) and 457–3 (lane b). Antibody 457–3 (which is specific for radixin) recognizes a single band corresponding to radixin. Antibody 220 (which binds to all three ERM proteins) recognizes two bands with molecular weights corresponding to radixin and moesin. DRG neuronal lysates do not appear to express ezrin. (B) DRG-dissociated neurons were grown on laminin-poly-l-lysine–coated coverslips and were fixed and probed with 457–3 anti-radixin followed by FITC-labeled anti-rabbit IgG. Radixin is present throughout the growth cone and exhibits a punctate pattern. Staining was undetectable for controls that lack 457–3.