Figure 3.

Micro-CALI using MG-nonimmune IgG has no effect. A chick DRG neuron was loaded with MG-labeled nonimmune IgG and plated on laminin-coated coverslip to allow a growth cone to emerge. Time-lapse imaging was begun at t = −5 min (A). The growth cone was subjected to laser irradiation within a small region of the growth cone (white circle) beginning at t = 0 min (B) andending at t = 5 min (C), and the growth cone was observed for another 10 min (D and E). The lamellipodia within the irradiated region do not retract (B–E). Scale bar, 10 μm.qending at t = 5 min (C), and the growth cone was observed for another 10 min (D and E). The lamellipodia within the irradiated region do not retract (B–E). Scale bar, 10 μm.