Figure 4.

Micro-CALI of radixin in the central region causes growth cone splitting. A chick DRG neuron was loaded with MG-labeled 457–3 and plated on a laminin-coated coverslip to allow agrowth cone to emerge. Time-lapse imaging was begun at t = −5 min (A). The growth cone was subjected to laser irradiation in its central region (white circle) beginning at t = 0 min (B) and ending at t = 5 min (C), and the growth cone was observed for another 10 min (D and E). The lamellipodia in the irradiated region retract (B and C), and the growth cone splits into two small growth cones (D and E). Similar results were seen using MG-labeled 220 in place of MG-labeled 457–3 (our unpublished results). No effect was seen with MG-labeled nonimmune IgG (see Table 2). Scale bar, 10 μm.