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Subcellular localization of functional Cnm67p fusion proteins visualized by fluorescence microscopy. (A) Cnm67-GFPp fluorescence is visible at one or two spots at the nuclear periphery, consistent with a localization to the SPB. Nuclear DNA was stained with DAPI. (B) Immunofluorescence microscopy of Cnm67–3HAp–labeled cells. Dot-shaped staining with anti-HA antibody coincides with the spindle poles visualized by tubulin staining with anti-tubulin antibody.
