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. 1998 May;9(5):977–991. doi: 10.1091/mbc.9.5.977

Figure 2.

Figure 2

Cnm67p coiled-coil prediction, tetrad analysis, and complementation test. (A) Probability plot for coiled-coil formation of the 581 amino-acid sequence of Cnm67p. The central part of the protein was predicted to contain three α helical coiled-coil regions (residues 174–226, p = 1; residues 306–357, p = 1; residues 382–448, p = 0.924) by the Paircoil program (Berger et al., 1995). The CNM67 sequence is available from EMBL/GenBank/DDBJ under accession number Z71501. (B) Tetrad dissection of a heterozygous CNM67-deleted strain (ABY101). Tetrad colonies were transferred on G418 containing medium to test for segregation of the kanMX4 gene deletion marker. Slow growing colonies always carried the cnm67::kanMX4 allele (cnm67Δ1). (C) Complementation of the cnm67Δ1 growth defect by transformation with a plasmid carrying the CNM67 wild-type allele.