Figure 3.

Nuclear distribution and microtubule structure in wild-type and cnm67Δ1. Cells were double stained for nuclear DNA with DAPI and for tubulin with anti-tubulin antibody. (A) Wild-type. (B) cnm67Δ1 cells. Panels B2 and B3 show different focal planes of the respective cells to visualize the complete microtubular structures. B1 and B2 show cytoplasmic microtubules that direct from one spindle pole into the bud. The spindle in B1 is highly elongated although it is restricted to the mother cell. Panel B3 depicts multinucleated cells. Bars, 10 μm.