Table 2.
Yeast strains
| Name | Genotype | Source or reference |
|---|---|---|
| FY1679 | MATα/a ura3-52/ura3-52 trp1Δ63/TRP1 leu2Δ1/LEU2 his3Δ200/HIS3 | B. Dujon, diploid from cross FY23 × FY73 (F. Winston) |
| CEN.PK2 | MATα/a ura3-52/ura3-52 trp1-289/trp1-289 leu2-3,112/leu2-3,112 his3Δ1/his3Δ1 | K.D. Entian |
| ABY99 | MATα cnm67Δ1::kanMX4 ura3-52 trp1-289 leu2-3,112 his3Δ1 | Brachat et al.a |
| ABY101 | MATα/a cnm67Δ1::kanMX4/CNM67 ura3-52/ura3-52 trp1Δ63/TRP1 leu2Δ1/LEU2 his3Δ200/HIS3 | Brachat et al.a |
| ABY102 | MATα cnm67Δ1::kanMX4 ura3-52 TRP1 leu2Δ1 his3Δ200 | Brachat et al.a |
| ABY103 | MATa cnm67Δ1::kanMX4 ura3-52 trp1Δ63 leu2Δ1 HIS3 | Brachat et al.a |
| ABY104 | MATa cnm67Δ1::kanMX4 ura3-52 trp1Δ63 leu2Δ1 his3Δ200 | Brachat et al.a |
| ABY105 | MATα/a cnm67Δ1::kanMX4/CNM67 ura3-52/ura3-52 trp1-289/trp1-289 leu2-3, 112/leu2-3,112 his3Δ1/his3Δ1 | This study |
| ABY106 | MATa cnm67Δ1::kanMX4 ura3-52 trp1Δ63 leu2Δ1 his3Δ200 {pYCGNL225c}b | This study |
| ABY107 | MATα/a cnm67Δ1::kanMX4/cnm67Δ1::kanMX4 ura3-52/ura3-52 trp1Δ63/TRP1 leu2Δ1/leu2Δ1 his3Δ200/HIS3 | This study |
| ABY108 | MATα/a cnm67Δ1::kanMX4/cnm67Δ1::HIS3MX6 ura3-52/ura3-52 trp1-289/trp1-289 leu2-3,112/leu2-3,112 his3Δ1/his3Δ1 | This study |
| ABY109 | MATα/a CNM67::GFP-kanMX6/CNM67 ura3-52/ura3-52 trp1-289/trp1-289 leu2-3,112/leu2-3,112 his3Δ1/his3Δ1 | This study |
| ABY110 | MATα CNM67::GFP-kanMX6 ura3-52 trp1-289 leu2-3,112 his3Δ1 | This study |
| ABY111 | MATa CNM67::GFP-kanMX6 ura3-52 trp1-289 leu2-3,112 his3Δ1 | This study |
| ABY112 | MATα/a CNM67::GFP-kanMX6/CNM67::GFP-kanMX6 ura3-52/ura3-52 trp1-289/trp1-289 leu2-3,112/leu2-3,112 his3Δ1/his3Δ1 | This study |
| ABY132 | MATα/a CNM67::HA-kanMX6/CNM67::HA-kanMX6 ura3-52/ura3-52 trp1-289/trp1-289 leu2-3,112/leu2-3,112 his3Δ1/his3Δ1 | This study |
| DHY2 | MATα cnm67Δ1::kanMX4 HHF2::GFP-kanMX6 ura3-52 TRP1 leu2Δ1 his3Δ200 | This study |
| DHY3 | MATa cnm67Δ1::kanMX4 HHF2::GFP-kanMX6 ura3-52 trp1Δ63 LEU2 HIS3 | This study |
| ABY134 | MATα HHF2::GFP-kanMX6 ura3-52 trp1Δ63 HIS3 LEU2 | This study |
| ABY135 | MATa HHF2::GFP-kanMX6 ura3-52 TRP1 leu2Δ1 his3Δ200 | This study |
| 5105 | MATα/a act1-4/act1-4 ura3/ura3 LEU2/leu2 HIS3/his3 | Palmer et al., 1992 |
| DBY2023 | MATa tub2-401 ura3-52 his4-539 lys2-801 | Huffaker et al., 1988 |
| DBY1384 | MATa tub2-104 ura3-52 his4-539 | Thomas et al., 1985 |
| YJC1372 | MATα/a act5Δ1::HIS3/act5Δ1::HIS3 ura3-52/ura3-52 trp1Δ63/trp1Δ63 leu2Δ1/leu2Δ1 his3Δ200/his3Δ200 lys2-801/lys2-801 ade2-101/ade2-101 | J. Cooper (Washington University) |
a
Manuscript in preparation.
b
pYCGNL225c is a pRS416 (Sikorski and Hieter, 1989) derivative containing CNM67 with promoter and terminator sequences.