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. Author manuscript; available in PMC: 2009 Apr 1.
Published in final edited form as: Curr Opin Immunol. 2008 Apr 21;20(2):149–157. doi: 10.1016/j.coi.2008.03.014

Figure 2. Resolution of developing and mature subpopulations of splenic B cells.

Figure 2

Splenocytes from a single 8-week old female C57BL/6 mouse were stained in a single tube with antibodies to the indicated cell surface molecules before analysis of 200,000 events on an LSRII flow cytometer. All non-viable cells were eliminated from this analysis by gating on cells that failed to stain with the UV-excited DNA dye DAPI, and myeloid-lineage cells were eliminated by gating on CD11b− Gr-1− cells (gates not shown). CD19+ cells were identified as shown (left-most plot) and three gating strategies were employed to identify the indicated subsets. Gating strategies are from Cariappa et al. (top) (30), Martin and Kearney, and Srivastava et al., (middle) (29, 31), or Allman et al., (bottom) (26).