SDS-PAGE (12%) analysis of purified recombinant apoA-I forms. (A) Proteins in lanes 1-5 are apoA-I forms produced by the baculovirus expression system and purified after cleavage of the His tag: lane 1, WT; lane 2, apoA-I[R160V/H162A]; lane 3, apoA-I[R116V/K118A]; lane 4, apoA-I[D102A/D103A]; lane 5, apoA-I[Δ(61-78)]. (B) Proteins in lanes 2-4 are apoA-I forms produced by the adenovirus expression system and purified: lane 2, WT; lane 3, apoA-I[E110A/E111A]; lane 4, apoA-I[Δ(121-142)]. In lane 6, (A), and lane 1, (B), are molecular mass markers (masses in kilodaltons).