. Author manuscript; available in PMC: 2008 Sep 8.

Published in final edited form as: J Am Chem Soc. 2006 Dec 20;128(50):15948–15949. doi: 10.1021/ja065303t

Table 1.

Thermodynamic parameters of the Pin WW Domain Variants

Protein	T_m^a (°C)	m value ^b (kcal/(mol·M))	ΔG_f^b (kcal/mol)	ΔΔG_f (kcal/mol)
wt Pin	59.8	1.04	-3.3 ± 0.1	0
V22F/Y23F	45.9	0.84	-2.6 ± 0.1	0.7 ± 0.1^c
O-Pin	27.6	1.51	-1.8 ± 0.1	0.8 ± 0.1^d
E-Pin	20.0	1.55	-1.3 ± 0.1	1.3 ± 0.1^d

calculated by fitting thermal denaturation curves.

calculated by fitting chaotrope denaturation curves.

For the control protein, ΔΔG_f = ΔG_f,V22F/Y23F - ΔG_f,wt.

For the O-Pin and E-Pin, ΔΔG_f = ΔG_f,mut - ΔG_f,V22F/F23F.