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. Author manuscript; available in PMC: 2008 Dec 18.
Published in final edited form as: Biochemistry. 2007 Nov 17;46(50):14450–14460. doi: 10.1021/bi701310x

Fig 3. Direct Binding of rFXIac to the Activated Platelet Surface.

Fig 3

Increasing concentrations of 125I-rFXIac were incubated with TRAP (25 μM) activated platelets (1–2 × 108 platelets/ml) in the presence of CaCl2 (2 mM) and ZnCl2 (25 μM). Non-specific binding was measured and subtracted from the total binding. Shown here is the specific binding of rFXIac to activated platelets with a Bmax of 270 ± 17; KD of 8.1 ± 1.49 nM. Values represent the mean ± standard deviation of three determinations performed in triplicate.