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. Author manuscript; available in PMC: 2008 Dec 18.
Published in final edited form as: Biochemistry. 2007 Nov 17;46(50):14450–14460. doi: 10.1021/bi701310x

Fig 4. Displacement of rFXIac from the Activated Platelet Surface by FXI and FXIa.

Fig 4

125I-rFXIac was incubated with non-radiolabeled FXI and FXIa and with platelets (1–2 × 108platelets/ml) and TRAP (25 μM) in the presence of CaCl2 (2 mM) and ZnCl2 (25 μM). FXIa (●) and not FXI (■) was able to displace rFXIac from the platelet surface with a Ki of 1.3 ± 0.16 nM, similar to that of rFXIac displacement of FXIa (Ki = 3.5 ± 0.42 nM). The values represent the mean ± standard deviation of three independent experiments each done in triplicate.