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ESI/FT MS/MS interrogation of thioether formation in His6-LctA during LctM catalysis. A) Expected cyanylation products. B) Cyanylation of the assay products after 40 min. Colored asterisks depict oxidation products, M+(O), of the identically colored parent ions. C) MS/MS analysis of the LctA-3 H2O species from Figure 1B (40 min). D) Fragment ion map of the LctA-3 H2O species.
