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. 1986 Sep;59(3):761–763. doi: 10.1128/jvi.59.3.761-763.1986

Negative regulation of early polyomavirus expression in mouse embryonal carcinoma cells.

C Cremisi, C Babinet
PMCID: PMC253258  PMID: 3016339

Abstract

Embryonal carcinoma cells are resistant to infection by polyomavirus (Py). We showed that this block was partially removed by inhibiting protein synthesis temporarily. The block was also partially removed when Py was coinfected with simian virus 40. Cycloheximide treatment of cells infected with Py mutants able to grow on PCC4 embryonal carcinoma cells led to 3- to 10-fold increases in the production of T-antigen-positive cells. At 31 degrees C, Py T-antigen expression was enhanced when the cells were treated with cycloheximide. We suggest that a negative labile regulatory protein(s) is synthesized in PCC4 cells, preventing the initiation of early Py transcription by binding to the noncoding sequence, especially the enhancer element B and perhaps also element A, and that the Py mutants retained a binding site(s).

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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