. Author manuscript; available in PMC: 2008 Sep 8.

Published in final edited form as: J Proteome Res. 2007 Mar 28;6(5):1735–1744. doi: 10.1021/pr060497x

Table 1.

To evaluate the variability of iTRAQ ratios induced by using a gel-based procedure, four aliquots of a protein mixture were analyzed in parallel. iTRAQ ratios for proteins from each of the four lanes are tabulated. Protein score >2.0 identified proteins with >99%. (The theoretical ratios between the samples were 1:1:1:1)

				Ratio
Protein ID	Accession #	# Pept	Score	115:114 (95% CI)	116:114 (95% CI)	117:114 (95% CI)
transferrin	gi\|31415705	37	77.2	1.2 (1.2; 1.3)	1.2 (1.2; 1.3)	1.1 (1.0; 1.1)
albumin 1	gi\|33859506	32	58.4	1.5 (1.4; 1.5)	1.6 (1.5; 1.7)	1.4 (1.3; 1.5)
chain A, Myoglobin	gi\|39654267	9	23.7	1.6 (1.4; 1.8 )	1.4 (1.1; 1.7)	0.68 (0.57; 0.81)
beta casein-bovine	gi\|226030	4	7.1	1.6 (1.2; 2.0)	1.7 (1.5; 2.0)	0.85 (0.67; 1.1)