. Author manuscript; available in PMC: 2008 Sep 8.

Published in final edited form as: J Proteome Res. 2007 Mar 28;6(5):1735–1744. doi: 10.1021/pr060497x

Table 2.

Analysis of the variability induced by the albumin depletion step by analyzing four aliquots of a protein mixture in parallel starting with depletion of albumin and then SDS-PAGE separation. iTRAQ ratios for proteins in slices cut out from the gel are tabulated. Protein scores >2.0 identified proteins with >99% confidence. (The theoretical ratios between the samples were 1:1:1:1)

				Ratio
Protein ID	Accession #	# Pept	Score	115:114 (95% CI)	116:114 (95% CI)	117:114 (95% CI)
transferrin	gi\|31415705	34	71.6	1.1 (1.1; 1.2)	0.82 (0.76; 0.88)	0.88 (0.83; 0.93)
chain A, Myoglobin	gi\|39654267	9	20.8	1.1 (1.0; 1.3)	0.81 (0.69; 0.94)	0.92 (0.81; 1.0)