Figure 8.

PS-ON patterns were stable over a period of 6.5 h. HeLa cells received 0.25 μM PS-ON 11068-F complexed to Lipofectin. Six hours after lipofection time-lapse microscopy of living cells was started and fluorescence images were taken at the indicated times from the same two cells. The arrowheads point to three particular PS bodies in the nucleus that were held in focus throughout the experiment. Their location with respect to each other and to other nuclear structures like the nucleoli did not change noticeably. Note that apparent changes in the PS body pattern, especially in the upper cell, are solely due to focus changes caused by the movements of the cell, e.g. rotations of the nucleus. Bar, 5 μm.