Fig. 3.

The time course of MCP-1 secretion in mouse peritoneal macrophages infected with T. gondii by ELISA (A). The macrophages were infected with T. gondii tachyzoite (•) or left untreated (◦) for 0, 6, 12 and 24 hr. The macrophages were pretreated with 20 µM PD98059 (▫) or 10 µM SP600125 (▪) for 20 min before infection. MCP-1 secretion levels of mouse macrophages were reduced by treatment with MAPK inhibitors (B). The macrophages were pretreated with ERK1/2 inhibitor (5 µM, 10 µM or 20 µM PD98059), p38 inhibitor (5 µM, 10 µM or 20 µM SB203580), JNK inhibitor (5 µM, 10 µM or 20 µM SP600125) or 10 µM mixed MAPK inhibitors for 20 min, and culture supernatants were obtained after 24 hr of infection. Data are expressed as the mean ± SD of 3 separate experiments. h = hour. None; mouse macrophages only, T. gondii; T. gondii infection. ^*P < 0.05.