Fig. 4.

Time course of MIPP-1α secretion in mouse peritoneal macrophages infected with T. gondii via ELISA (A). The macrophages were cultured with T. gondii (•) or left untreated (◦) for 0, 6, 12 and 24 hr. The macrophages were pretreated with 20 µM PD98059 (▫) or 10 µM SP600125 (▪) for 20 min, and were infected with T. gondii. The MIPP-1α secretion levels of the macrophages were reduced by treatment with ERK1/2 or JNK MAPK inhibitors (B). The macrophages were pretreated with 5 µM, 10 µM or 20 µM PD98059, 5 µM, 10 µM or 20 µM SB203580, 5 µM, 10 µM or 20 µM SP600125, or 10 µM of all 3 MAPK inhibitors for 20 min, and were infected with T. gondii for 24 hr. MIPP-1α mRNA was upregulated in RAW 264.7 cells infected with T. gondii, according to the results of RT-PCR analysis (C). RNA was extracted from RAW 264.7 cells at 1, 3, 6, and 18 hr PI with T. gondii. RAW 264.7 cells were pretreated with 20 µM PD98059, 10 µM SB203580 or 10 µM SP600125 for 20 min, and were infected with T. gondii for 18 hr. Data are expressed as the mean ± SD of 3 separate experiments (A & B). h = hour. None (B); mouse macrophages only, T. gondii; T. gondii infection. ^*P < 0.05. None (C); RAW 264.7 cells only, PD; PD98059, SB; SB203580, SP; SP600125.