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SDS-PAGE analysis of the recombinant hemoglobinase expressed in E. coli. Lanes: M, molecular mass standards; 2, lysates of BL21; 2, pGEX-4T1; 3, pGEX-4T1/hemoglobinase; 4, purified recombinant hemoglobinase (arrow indicates GST-hemoglobinase and arrow head indicates purified P. westermani hemoglobinase).
