Figure 9.

Effects of Kihi-to on axonal extension following Aβ(25–35)-induced atrophy. Cortical neurons were cultured for 4 days and then treated with or without (Cont) 10 μM Aβ(25–35). Four days after the administration of Aβ(25–35), the cells were treated with Kihi-to (0.01 and 0.1 μg/ml), 100 ng/ml of NGF, or vehicle (Veh). Five days after treatment, the cells were fixed and immunostained with an antibody against phosphorylated NF-H (A). The lengths of NF-H-positive neurites (B) were quantified for each treatment. *p < 0.05 vs. Veh, n = 4 (one-way ANOVA followed by Holm-Sidak post hoc test). Scale bar = 100 μm.