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. 2008 Aug 12;8:63. doi: 10.1186/1472-6750-8-63

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Copyright © 2008 Minaeva et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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New φ80-cognate CRIM plasmids with λ-removable part. A. Map of pAH162-λattL-Tc^R-λattR. This plasmid could be used as a vector for molecular cloning of the genes of interest followed by φ80-Int-dependent integration of the recombinant plasmid in bacterial chromosome and λ-Int/Xis-dependent excision of the selective marker-carrier vector part. B. Map of pAH162-λattL-Tc^R-λattR-Cm^R. The recombinant plasmid constructed on the basis of new CRIM-vector, that contains cat-gene under the transcriptional control of phage T7 A2-promoter, as a model gene for integration in the chromosome according to Dual-In/Out strategy.