Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Western blot confirming the monospecificity of the antibodies used. Only bands at the expected position are observed with antibodies against the following proteins: PTFγ, 45 kDa (lane 1); TBP, 44 kDa (lane 2); RNA polymerase II large subunit, 240 kDa (lane 3); TFIIH p62, 62 kDa (lane 4); TFIIF RAP74, 74 kDa (lane 5); and p80-coilin, 80 kDa (lane 6). The antibody 8WG16 against the RNA polymerase II large subunit (lane 3) labels multiple bands due to phosphorylation of the protein.
