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. 2008 Jul 21;36(16):e103. doi: 10.1093/nar/gkn398

Figure 1.

Figure 1.

Liposome-mediated transfection of plasmids that encode either a ZF-TF or LacZ into HEK 293 cells and HM7 cancer cells. HEK 293T cells (A) and HM7 human cancer cells (B) were transfected with a plasmid that expressed F435-KRAB or the LacZ gene product, β-galactosidase (pcDNA3.1/His/LacZ; Invitrogen) using Lipofectamine 2000 (Invitrogen) and the amounts of secreted VEGF-A were measured by ELISA at 2 days after transfection. The F435-KRAB-encoding plasmid efficiently suppressed the expression of VEGF-A in 293T cells, but not in HM7 cells. (C and D) HEK 293T cells and HM7 cells transfected with the LacZ plasmid were stained to visualize the enzyme activity. Greater than 90% of the transfected 293T cells were LacZ positive, but <1% of the HM7 cells were LacZ positive.