Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2008 Aug 20;36(16):5417–5425. doi: 10.1093/nar/gkn528

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2008 The Author(s)

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Figure 2. — Base flipping by PspGI enzymes. (A) Fluorescence of 2AP26 duplex (500 nM) under different conditions. Measurements were done in the MTase buffer without SAM or its analogs. MTase refers to M.PspGI (1.5 μM) and the divalent metal ions were added as chloride salts at 10 mM. The results shown are mean and SD from three parallel measurements made at 65°C. (B) Lack of cofactor requirement for base flipping by M.PspGI. Incubation conditions were similar to those in part A except no divalent metals were added. Instead SAM or SAH were added as indicated. The results shown are mean and SD from three measurements made at 65°C.