Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
2AP fluorescence enhancement by R.PspGI. The fluorescence of 2AP18 duplex (2.25 μM in REase buffer with 10 mM CaCl2) was monitored at 55°C with time in two cuvettes in parallel. At 5 min, WT R.PspGI was added to one cuvette to final concentration 5 μM (upper curve) and fluorescence measurements were continued. At 15 min, MgCl2 was added to both the cuvettes to final concentration of 10 mM and measurements were continued for additional 45 min.
