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Dependance of R.PspGI catalytic activity on temperature. The fluorescence of 2AP27 duplex (500 nM in REase buffer with 10 mM MgCl2) was monitored at various temperatures. The reactions were started with the addition of WT R.PspGI to concentration of 80 nM and fluorescence was monitored for a few minutes to a few hours depending on the temperature. The initial velocity of the reactions were calculated and normalized with respect to the velocity at 55°C.
