Table 1.
DNA oligomers
| Name | Sequencea | Melting temperature of duplexb (°C) |
|
|---|---|---|---|
| REase bufferc | MTase bufferd | ||
| 2AP18 | TGCTACC2GGC GAAGATA-biotin | 67 | – |
| O15N | CTTCGCCTGGTAGCA | – | – |
| 2AP21 | CGACGCAAGCCGACGCCA GC2CCACCAGGACGCCGCATA | 87 | 75 |
| 2AP26 | CGACGCAAGCCGACGCCAGCAC CAC2AGGACGCCGCATA | 85 | 71 |
| 2AP27 | CGACGCAAGCCGACGCC AGCACCACC2GGACGCCGCATA | 87 | 75 |
| O2APT | GCGGCGTCCTGGTGGTGCTG GCGTCGGCTTGCGTCG | – | – |
aPosition of 2AP in the sequence is indicated by 2.
bMelting temperatures of duplexes containing a 2AP-containing oligomer hybridized to their complement (i.e. 2AP18 with O15N, and 2AP21, 2AP26 or 2AP27 with O2APT). The melting temperature is the midpoint of 2AP fluorescence enhancement when the temperatures of duplexes were increased from 25°C to 95°C in the absence of any protein.
cREase buffer—10 mM Tris pH 7.9, 50 mM NaCl, 10 mM MgCl2.
dMTase buffer—10 mM Tris, pH 8.0, 1 mM EDTA.