Figure 3.

Effect of RecUK56A or RecUR71A on RecA catalyzed dATP hydrolysis. Circular ssDNA (10 μM) was preincubated with RecA (1.3 μM, in (A)) or with RecU variants (200 nM, in (B)) in buffer A containing 2 mM dATP for 5 min at 37°C. Then an RecU variant was added to RecA (A) or RecA was added to the RecU variants (B), and the reaction mixtures were incubated for further 25 min at 37°C. Aliquots were taken and the extent of RecA catalyzed hydrolysis of dATP is shown in pmols. The arrow indicates the addition of the second protein. Values are averages of four independent experiments.