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. 2008 Aug 6;36(16):5242–5249. doi: 10.1093/nar/gkn500

Figure 5.

Figure 5.

RecUK56A or RecUR71A does not interact with RecA. (A and B), RecUWT, RecUK56A, RecUR71A (2 μM) or RecA (2 μM) or combinations of them were incubated with 50 μM DSS for 10 min. Immunoblot of cross-linked proteins using anti-RecA (A), or anti-RecU antibodies (B). The novel 62 kDa band is indicated by AU. An and Un denote the predicted state of the given protein (e.g. A1, A2, A3 indicates the oligomeric state of RecA). Plus and minus denote the presence and absence of the indicated protein, and/or cross-linking agent. The position of the molecular mass marker (in kDa) is shown.